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contour-clamped homogeneous electric field (chef-mapper) apparatus  (Bio-Rad)


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    Structured Review

    Bio-Rad contour-clamped homogeneous electric field (chef-mapper) apparatus
    Contour Clamped Homogeneous Electric Field (Chef Mapper) Apparatus, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/contour-clamped+homogeneous+electric+field+(chef)+mapper+system/contour+clamped+homogeneous+electric+field+mapper/pmc04193765-99-18-25
    Average 90 stars, based on 1 article reviews
    contour-clamped homogeneous electric field (chef-mapper) apparatus - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Electrophoresis:

    Article Title: Phenotypic and molecular characteristics of Escherichia coli isolated from aquatic environment of Bangladesh.
    Article Snippet: .. The plugs were successively washed twice with water and four times with TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 8.0) for 15 min each at 54 C. The agarose plugs were equilibrated in an appropriate restriction enzyme buffer for 1 hr at 37 C and the embedded DNA was digested overnight at 37 C with 50 U of XbaI from TaKaRa Bio, Inc. Electrophoresis was performed using the autoalgorithm mode of contour clamped homogeneous electric field Mapper system (Bio-Rad) to resolve the DNA size range of 20 to 350 kb with 1% PFGE grade agarose (Bio-Rad) at 6 V/cm for 40 hr 24 min at 14 C. After electrophoresis, the gel was stained with ethidium bromide and the gel image was captured digitally in a gel documentation system (Gel Doc 2000, Bio-Rad). .. A lambda ladder (New England Biolabs, Mass., U.S.A.) was used as the molecular size standard.

    Staining:

    Article Title: Phenotypic and molecular characteristics of Escherichia coli isolated from aquatic environment of Bangladesh.
    Article Snippet: .. The plugs were successively washed twice with water and four times with TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 8.0) for 15 min each at 54 C. The agarose plugs were equilibrated in an appropriate restriction enzyme buffer for 1 hr at 37 C and the embedded DNA was digested overnight at 37 C with 50 U of XbaI from TaKaRa Bio, Inc. Electrophoresis was performed using the autoalgorithm mode of contour clamped homogeneous electric field Mapper system (Bio-Rad) to resolve the DNA size range of 20 to 350 kb with 1% PFGE grade agarose (Bio-Rad) at 6 V/cm for 40 hr 24 min at 14 C. After electrophoresis, the gel was stained with ethidium bromide and the gel image was captured digitally in a gel documentation system (Gel Doc 2000, Bio-Rad). .. A lambda ladder (New England Biolabs, Mass., U.S.A.) was used as the molecular size standard.

    other:

    Article Title: Molecular signatures of aneuploidy-driven adaptive evolution
    Article Snippet: PFGE was conducted using a BioRad Contour-clamped homogeneous electric field (CHEF) Mapper XA system and genomic samples were prepared using the CHEF genomic DNA plug kit.



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